Proteomic and Lipidomic Changes During Insulin Granule Aging
Endocrine cells utilize secretory granules for the storage and regulated release of peptide- hormones and neurotransmitters - a process influenced by spatiotemporal factors like the proximity of these organelles to the plasma membrane and their age. While recent progress has been made, a comprehensive view about the molecular composition and aging-related changes of the insulin-containing secretory granules (ISGs) of pancreatic islet beta cells remains elusive. Now, new light was shed on the molecular composition of ISGs, which is relevant to better understand their behavior in healthy conditions and possibly in diabetes, in which insulin release is impaired.
A team of researchers from the Paul Langerhans Institute Dresden (PLID) of the German Center for Diabetes Research, the Center of Membrane Biochemistry and Lipid Research at TU Dresden, the Max Planck Institutes for Biochemistry and Medical Research, respectively, as well as the Center for Molecular and Cellular Bioengineering have unveiled a novel immunobased approach for the purification of ISGs. Published in renowned Journal “Cell Reports”, this refined protocol enabled for the first time the accurate proteomic and lipidomic profiling of age-distinct ISG pools.
